East African cassava mosaic virus‐Uganda (EACMV‐UG) and African cassava mosaic virus (ACMV) reported for the first time in Central African Republic and Chad

Occurrence of African cassava mosaic virus (ACMV) and East African cassava mosaic virus – Uganda (EACMV-UG) in Jatropha curcas

Background Jatropha curcas is a drought resistant shrub native in tropical America, now widely grown in many tropical and subtropical regions for biodiesel production [1]. First reports on virus infections in Jatropha indicated the occurrence of viruses closely related to Cassava mosaic virus in India, reaching a disease incidence from 25 to 47%. This might represent a major constrain to the pr...

Geminate structures of African cassava mosaic virus.

Two types of geminate structures were purified from African cassava mosaic geminivirus (ACMV)-infected Nicotiana benthamiana plants and analyzed by electron cryomicroscopy and image reconstruction. After cesium sulfate density gradient centrifugation, they were separated into lighter top (T) and heavier bottom (B) components. T particles comigrated with host proteins, whereas B particles were c...

Nucleotide sequence of the infectious cloned DNA components of African cassava mosaic virus (Nigerian strain)

RNA-primed complementary-sense DNA synthesis of the geminivirus African cassava mosaic virus.

The plant DNA virus African cassava mosaic virus (ACMV) is believed to replicate by a rolling circle mechanism. To investigate complementary-sense DNA (lagging strand) synthesis, we have analysed the heterogenous form of complementary-sense DNA (H3 DNA) from infected Nicotiana benthamiana by two-dimensional agarose gel electrophoresis and blot hybridisation. The presence of an RNA moeity is dem...

Properties of African Cassava Mosaic Virus Capsid Protein Expressed in Fission Yeast

The capsid proteins (CPs) of geminiviruses combine multiple functions for packaging the single-stranded viral genome, insect transmission and shuttling between the nucleus and the cytoplasm. African cassava mosaic virus (ACMV) CP was expressed in fission yeast, and purified by SDS gel electrophoresis. After tryptic digestion of this protein, mass spectrometry covered 85% of the amino acid seque...